Transcriptomic difference in bovine blastocysts following vitrification and slow freezing at morula stage

Transcriptomic difference in bovine blastocysts following vitrification and slow freezing at morula stage

Cryopreservation is known for its marked deleterious effects on embryonic health. Bovine compact morulae were vitrified or slow-frozen, and post-warm morulae were cultured to the expanded blastocyst stage. Blastocysts developed from vitrified and slow-frozen morulae were subjected to microarray analysis and compared with blastocysts developed from unfrozen control morulae for differential gene ...

Clinical outcomes following cryopreservation of blastocysts by vitrification or slow freezing: a population-based cohort study.

STUDY QUESTION What are the clinical efficacy and perinatal outcomes following transfer of vitrified blastocysts compared with transfer of fresh or of slow frozen blastocysts? SUMMARY ANSWER Compared with slow frozen blastocysts, vitrified blastocysts resulted in significantly higher clinical pregnancy and live delivery rates with similar perinatal outcomes at population level. WHAT IS KNOW...

Different chromatin and energy/redox responses of mouse morulae and blastocysts to slow freezing and vitrification

BACKGROUND The ability to cryopreserve mammalian embryos has become an integral part of assisted reproduction, both in human and veterinary medicine. Despite differences in the size and physiological characteristics of embryos from various species, the embryos have been frozen by either of two procedures: slow freezing or vitrification. The aim of our study was to compare the effect of slow fre...

The Effect of Pestivirus on the in vitro Development of Bovine Morula and Blastocysts.

In vitro development and gene expression of frozen-thawed 8-cell stage mouse embryos following slow freezing or vitrification

OBJECTIVE This study was performed to compare the efficiency of slow freezing and vitrification based on survival, development to blastocysts, and cell numbers of blastocysts. Changes in embryonic gene expression in fresh and frozen-thawed embryos were also examined. METHODS Eight-cell stage embryos were collected from superovulated female BDF1 mice. The collected embryos were randomly divide...